Assessing the Performance Capabilities of LRE-Based Assays for Absolute Quantitative Real-Time PCR
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چکیده
منابع مشابه
Assessing the Performance Capabilities of LRE-Based Assays for Absolute Quantitative Real-Time PCR
BACKGROUND Linear regression of efficiency or LRE introduced a new paradigm for conducting absolute quantification, which does not require standard curves, can generate absolute accuracies of +/-25% and has single molecule sensitivity. Derived from adapting the classic Boltzmann sigmoidal function to PCR, target quantity is calculated directly from the fluorescence readings within the central r...
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ژورنال
عنوان ژورنال: PLoS ONE
سال: 2010
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0009731